To examine the effects of vaccination against Babesia gibsoni(B gibsoni) infection in dogs, 15 normal mixed-breed dogs(5 months to 1 year old) were divided into 3 groups with 5 dogs in each group. One of them was selected as control group (group A)and other were selected as experimental groups (group B and C).
The group B was vaccinated with antigen which were mixed 0.2% of formalin treated B gibsoni and sonicated one. The group C was inoculated Theileria sergenti as a non-specific antigen.
The results obtained in this experiment were summarized as follows ;
1. After first vaccination, antibody titers of group B md C were increased 5 times(1 : 200) than those of control group(1 : 40). The antibody titers of group C; were increased more than that of group B after second vaccination. When challenged with the living protozoa (Babesia gibsoni) , the antibody titers of C group were elevated higher than that of B group and maintained steadly. Those were not exceeded over 1 : 5, 000 for 4 weeks in all 3 groups.
2. After challenge, the peak time of the parasitemia appeared nearly on the 15th day (12∼18 days ) in all groups. During this period, the rate of parasitized erythrocytes in control group was 55.0±5.4‰. Bnt that of group B and C were 41.3±38.8‰ and 15.2±16.3‰, respectively.
3. After challenge with B gibsoni, all of the values of YCV, Hb, RBC and total leukocytes counts were decreased in both of the experimental and the control.
4. In all groups, there were increased lymphocytes and monocytes after challenge with the protozoa.
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